The present study aims to examine the influence of entrepreneurial marketing and organizational climate on organizational ambidexterity within tourism startups. To enhance the generalizability of the findings, the research was conducted among tourism startups operating in Mazandaran Province, Iran. Methodologically, this study adopts a descriptive-survey design and is classified as applied research in terms of its objective. The statistical population encompasses all senior executives and senior experts affiliated with startup enterprises registered in Mazandaran Province. According to official records, approximately 28 startup companies are formally registered in the province, comprising an estimated population of 288 senior managers and experts. Based on Cochran's sample size formula, a representative sample of 165 participants was determined. A simple random sampling technique was employed to select respondents. Data were collected using a standardized questionnaire. To ensure instrument reliability, Cronbach's alpha coefficient was computed and confirmed to exceed acceptable thresholds. Data analysis and hypothesis testing were performed using SmartPLS 4 and SPSS statistical software packages. The empirical findings reveal that entrepreneurial marketing exerts a statistically significant positive effect on both exploration and exploitation dimensions of organizational ambidexterity among tourism startups in Mazandaran Province. Furthermore, organizational climate demonstrates a significant and positive influence on both exploration and exploitation capabilities within the same context. These results underscore the strategic importance of fostering entrepreneurial marketing practices and cultivating a supportive organizational climate to enhance ambidextrous capabilities in tourism-oriented startup ecosystems.